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CABRI:ICLC |
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Accession_number |
ICLC HTL03003
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Cell_line_name |
293
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Brief_description |
Species: human; Tissue: kidney, embryonic; Transformed by: adenovirus 5
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Description |
Species: human; Tissue: kidney, embryonic; Transformed by: adenovirus 5
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Depositor |
Obtained from ECACC
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Bibliographic_reference |
J Gen Virol 1977;36:59-74 [PMID: 886304
] |
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Morphology |
epithelial-like, grown as monolayer
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Culture_conditions |
continuous culture; DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Split cultures 1:3-1:6 using trypsin/EDTA; 37°C, 5% CO2. The viability may be low after thawing and the cells may take up to 7 days to attach. The cells should be frozen at not less than 8 x 10^6 /ampoule and thawed with 20% FBS
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Viruses |
Search for viruses was not performed
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
Handle as Biosafety Level 2 containment
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Freezing_medium |
90% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Validation_assays |
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
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Further_bibliography |
Virology 1977;77:319-329 [PMID: 841862
]
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